CITI INITIAL BIOSAFETY
PRACTICE TEST 94
QUESTIONS COMPLETE
Question 1: Which three elements form the primary framework for biosafety
containment in a laboratory?
Choices:
1) Work practices, safety equipment, and facility design 2) Vaccination, insurance, and licensing 3) Training, publication, and peer review 4) Inventory, procurement, and shipping
Correct Answer: Work practices, safety equipment, and facility design
Explanation: Biosafety containment is built from appropriate work practices, primary safety equipment such as biological safety cabinets, and laboratory facility design. These elements are selected through risk assessment.Page 1
Question 2: A laboratory works only with a well-characterized microorganism not
known to consistently cause disease in healthy adults. Which biosafety level is the usual starting point?
Choices:
1) BSL-4
2) BSL-3
3) BSL-1
4) BSL-2
Correct Answer: BSL-1
Explanation: BSL-1 is the usual starting point for work with well-characterized agents that are not known to consistently cause disease in healthy immunocompetent adults. A protocol- speci�c risk assessment can still require additional precautions.Question 3: At which biosafety level are Class II biological safety cabinets commonly used for procedures that may create infectious aerosols or splashes even though some work may be performed on an open bench after risk assessment?
Choices:
1) BSL-2
2) BSL-1
3) BSL-4
4) BSL-3
Correct Answer: BSL-2
Explanation: BSL-2 practices apply to agents associated with human disease that present moderate hazards. Aerosol- or splash-generating procedures are performed in a BSC or other appropriate containment based on risk assessment.Page 2
Question 4: Which feature most clearly distinguishes typical BSL-3 work from BSL-2 work?
Choices:
1) Food and drink are prohibited 2) A sink is available for handwashing 3) Personnel receive safety training 4) All procedures involving infectious materials are conducted within appropriate primary containment and the laboratory uses directional inward air�ow Correct Answer: All procedures involving infectious materials are conducted within appropriate primary containment and the laboratory uses directional inward air�ow Explanation: BSL-3 is designed for work with agents that may cause serious or potentially lethal disease through inhalation. Primary containment and controlled directional air�ow are key additional containment features.
Question 5: Which statement best describes BSL-4 containment?
Choices:
1) It is limited to nonpathogenic teaching strains 2) It is the highest containment level and may use Class III cabinets or positive-pressure suits in specially designed facilities 3) It never requires respiratory protection 4) It is identical to BSL-2 except for double gloves Correct Answer: It is the highest containment level and may use Class III cabinets or positive- pressure suits in specially designed facilities Explanation: BSL-4 is the highest laboratory containment level and is used for very high-risk agents. Work is performed in specialized facilities using Class III cabinets or positive-pressure suit laboratories with stringent engineering and procedural controls.Page 3